The acumen explorer laser scanning fluorescence microplate cytometer ttp labtech ltd, melbourn, united kingdom is a nonconfocal system, permitting areabased scanning of fluorescent objects in whole wells, without the need of a nuclear stain required by image platforms. The acumen tool suite takes project analysis and schedule optimization to the next level. Antisense inhibition of human mirnas and indications for. Ttp labtech limited presents acumen explorer, its laserscanning fluorescence microplate cytometer for highcontent screening.
The acumen optics, where the field of view is 20 mm 2, allow the entire well area to be rapidly imaged and processed, with whole plate read times of only 8 minutes. Download citation highcontent screening in oncology using fluorescence microplate cytometry ttp labtech limited presents acumen explorer, its laserscanning fluorescence microplate. Script to download file from labtech cloud server help. Latest developments in high content screening systems. Fluorescently labeled plates were analyzed with acumen explorer x3 ttp labtech, royston, uk. Through understanding more about compound mechanism of action, ttp labtechs acumen cellista offers an alternative approach that eliminates false positive. Oct 15, 2014 this should fix everything, but if scans do not run the make sure that the filelist. I agree to the use of cookies and have read and understood ttp labtechs privacy policy. Acumen qhts assay for inhibitors of the mtorc1 signaling. Cells treated with nonirritating concentrations of compounds and formulations take up calceinam and convert it to fluorescent calcein which is. The fluorescence emission from the alexa488 conjugated rps6 phospho235236 antibody staining is selected using a 530585 nm band pass filter.
Providing agility, efficiency and productivity for a broad range of applications. Aptly named high content analysis, such an approach is enabling new meth. Ly2801653 is an orally bioavailable multikinase inhibitor. Filter products all application categories drug discovery molecular biology and genomics structural biology sample management all applications assay development high throughput screening hittolead optimisation medicinal chemistry nextgeneration sequencing single cell genomics protein crystallography biostorage and biobanking small molecule. Instruments such as the acumen explorer ttp labtech can scan and analyze an entire 1536well plate in 10 minutes, thus maintaining the throughputs required while providing highcontent information. Enables rapid wholewell imaging of cells and large 3d objects labelled with up to 4 multiplexed fluorophores over an area of 18 x 18 mm. Determination of cell colony formation in a highcontent. Aug 23, 2006 the acumen ex3 microplate cytometer is the next generation of the acumen explorer highcontent screening system.
This high content screening system provides the utmost in speed and efficiency, simultaneously collecting and analyzing data throughout the entire well without sacrificing image resolution. It scans entire wells with up to 4 channels of simultaneous data collection. Students as young as eight years and up to the phd level use it to learn stem subjects. Application of laserscanning fluorescence microplate cytometry. Surflog collects the browser history for chrome, internet explorer, firefox and safari browsers and stores that data inside the labtech database. The cells were next counted using the acumen explorer ttp labtech. This will be a great opportunity to learn more about acumen and exchange knowledge with fellow users. Imagebased screening at three levels of magnification. Ttp labtech s acumen explorer fluorescence microplate cytometer enables high throughput primary and secondary screening of cellbased assays by delivering the object recognition of ccd imagers combined with the fast reads of bulk fluorescence readers.
The microwell plate was imaged in a single acquisition at low magnification. The plates were then analyzed on an acumen explorer ttp labtech. The iuvo chemotaxis assay plate was designed for high throughput screening assays, being compatible with liquid handlers and appropriate imaging equipment such as ttp labtech s acumen hci. Ttp labtech an evolution of its highly successful acumen explorer hcs platform technology, the acumen ex3 is equipped with up to three lasers at 405, 488 and 633 nm, offering a wavelength range for excitation that is similar to that of white light source instrumentation and significantly increasing the variety of fluorescent reagents compatible. Highcontent screening in oncology using fluorescence microplate.
Each data point represents three technical replicates. Feb 15, 2006 new data that shows the effectiveness of ttp labtech s acumen explorer for high content screening at high throughput reveals the advances high content screening has made in applications such as protein kinase activation, cell cycle analysis and betalactamase reporter gene analysis. Change in membrane integrity was measured by the addition of 2. Nuclei in each well were imaged and enumerated by an acumen explorer ex3 ttp labtech plate reader at 405nm excitation and 420 to 500nm emission. Accept learn more shop online careers applications. Been having issues with one of my old scripts to download a file from my labtech server which is being hosted in the cloud from labtech.
The ttp labtech acumen ex3 is a high throughput microplate cytometer that allows for the collection and analysis of more than 40 images per second. Acumen application note the coupling of ttp labtech. After treatment, the cells were washed, fixed and stained with antibodies to detect phosphoproteins and propidium iodide to monitor dna content. In vitro l6 irritation assay predicts clinical injection. The central image was scanned with a quasiconfocal imager at medium magnification and cells can be resolved as homogeneous targets without internal detail. Highcontent screening in oncology using fluorescence. We added a copy of file to zip download in case the script import fails to create it. Acumen fuse ensures a solid, reliable project schedule in minutes, while acumen risk eliminates the statistical and logical challenges of typical risk models. To reset your password, enter your email address in the box below and click reset password.
Most of these are highthroughput microscope vendors and license fees range from thousands to tens of thousands of dollars. Acumen an intuitive tool for simulating mathematical models of cyberphysical systems and for visualizing them as plots or in 3d. The relative number of cells was normalized against a negative control inhibitor, gapas, used in these experiments. Introduction to the quantitative analysis of twodimensional. It provides an extremely fast, sensitive and robust method to detect the effects of small molecules or rnais on signalling pathways, without the requirement for complex, prolonged offline image analysis.
Application of laserscanning fluorescence microplate. This one day event features a series of talks and discussions relevant to acumen based high content screening. Labtech ltd manufactures pc based ecg holter systems, abpm, netecg, and rest and stress test ecg products. The assay plates were then measured by a bottom reading mode in an acumen explorer reader ttp labtech inc. In your labtech script you can use a file write text script step to write the ps1 down to the remote computer and then use a shell command to execute it using powershell. Highthroughput multiplexed quantitation of protein. For the 1536well format used for screening, we measured gfp signal expressed by the cells as total fluorescence area m 2 in a well using an acumen explorer ttp labtech, and we reported growth rates as percent confluency of the gfpexpressing cells using a total well surface area of the bottom of the well as 100% confluence. The device is a laserscanning fluorescence microplate reader that enables the development and execution of cellbased high content. A nuclei population was defined by a 10 to 100 um width and depth filter that eliminated small and large fluorescent particles. To determine the number of a549 cells remaining following transfection with the mirna inhibitors, we stained the cells using viacount flex reagent and analyzed. Covering a wide range of consumables for spt labtech instruments and applications, including dragonfly discovery, mosquito hv genomics and others. We accelerate research through collaboration, deep application knowledge and worldleading engineering. Ttp labtech s european acumen user group meeting in heidelberg.
Laserbased 488nm fluorescent microplate cytometer enables whole plate reads in just 10 minutes regardless of plate type. It is equipped with multiple lasers, offering a wavelength range for excitation. Eliminate bottlenecks by moving simple, routine assays from high content imaging devices onto acumen, freeing up your instruments and operators to. The plates are scanned on the acumen explorer laser scanning instrument ttp labtech using the 488nm laser for simultaneous excitation of the alexa488 and pi.
The resolution of cellbased assays down to the cellular level has created new opportunities for the drug discovery process. Ttp labtechs european acumen user group meeting in heidelberg. This application note describes the use of an iuvo chemotaxis assay plate in combination with ttp labtech s acumen hci high content imager for the. Antisense inhibition of human mirnas and indications for an. The acumen hci laser scanning imager offers rapid whole well image acquisition regardless of plate type 24 to 1536well plates. We worked out a semiautomatic way to perform the immunostaining washing steps using the multidrop combi reagent dispenser thermo scientific. Ttp labtech s acumen ex3 microplate cytometer can read entire 384well plate in less than 10 minutes including the assay with other biomarkers. The instrument allows rapid, multiparametric analysis of. In vitro l6 irritation assay predicts clinical injection site. Ttp labtechs acumen explorer fluorescence microplate cytometer enables high throughput primary and secondary screening of cellbased assays by delivering the object recognition of ccd imagers combined with the fast reads of bulk fluorescence readers. The plates were read by the acumen explorer using a 1 x 8 um scan of the whole well area.
Aug 15, 2014 surflog collects the browser history for chrome, internet explorer, firefox and safari browsers and stores that data inside the labtech database. Bringing the benefits of a high content approach, without the bottlenecks. Selectscience trusted information for laboratory scientists. In this application note we demonstrate the use of fluorescent dyes to analyse the cell cycle phases of adherent hela cells in situ in 384well plates using ttp labtech s acumen hci, which provides valuable secondary information by preserving morphological changes that may have occurred during drug treatment. You should receive an email shortly after submitting your request. A chemical genomics screen to discover genes that modulate. Fuse produces clear and concise reports so analysis and performance can easily be reported to contractors along with todo lists of any needed improvements.
We have access to and expertise using ge incell analyzer 2200, ge incellanalyzer 6000, ttp labtech acumen explorer, bd pathway 435, licor odyssey imager, 10 tb and 30 tb dedicated image servers, incuctye. This should fix everything, but if scans do not run the make sure that the filelist. Browser versions posted on march 21, 2016 stemming from a request to make it simpler to view the version of internet explorer on computers, this plugin was born. Ttp labtech acumen cellista laser scanning imaging cytometer. Ttp labtech announce an exciting new development for its worldclass, high content, microplate imaging system, the acumen ex3. New data that shows the effectiveness of ttp labtechs acumen explorer for high content screening at high throughput reveals the advances high content screening has made in applications such as protein kinase activation, cell cycle analysis and. The latest generation of these instruments is the acumen explorer ttp labtech that provides read times sufficient for high. Accurately forecasting cost, completion and revenue all hinges on a solid, reliable project schedule.
Briefly, imagebased assays acumen explorer, tpp labtech were used for compound screening. Eliminate bottlenecks by moving simple, routine assays from high content imaging devices onto acumen, freeing up your instruments and operators to focus on more complex investigations. Cells were seeded in 96well dishes, allowed to attach overnight and then treated with test compounds for 24 h. Metamorph, imagepro plus, thermofisher cellomics, ge incell, perkinelmer evotec opera, molecular devices metaxpress, bd pathway atto, compucyte, ttp labtech acumen explorer, and definiens cellenger. Pi fluorescence triggered the collection of pi and gfp signals. The acumen explorer laser scanning fluorescence microplate cytometer ttp labtech ltd, melbourn, united kingdom is a nonconfocal system, permitting areabased scanning of fluorescent objects in whole wells, without the need of a. The x3 can analyze adherent cells in situ, unlike flow cytometry whi. Ttp labtech s acumen has been widely used to study protein phosphorylation. A new whollyowned subsidiary, ttp labtech, was created in 1997 to facilitate the sale of this proprietary product. Flow cytometry was also used to determine cell cycle distribution in cell lines, as described in the supplemental. You can then use a variable check script step to check the shell result for files extracted successfully. Automatically generate schedule scenarios and make smart acceleration a reality with acumen 360. Using an automated plate washer kalypsys, medium was aspirated from each well, leaving a residual volume of approximately 2.
Thus it took 10 years between the first small thirdparty industrial design contract and the launch of the acumen explorer, and a. The ic 50 and ec 50 values are determined by curve fitting to a 4parameter logistic for each output using activity base software. Preclinical characterization of the cdk46 inhibitor. Script to download file from labtech cloud server help labtech 11. Avoid delays, cut costs and measure performance with true project intelligence. The continuous tape model was a novel concept years ahead of its time, but the three product areas have remained. The explorer range, originally developed by ttp labtech and sold by acumen, has been refocused around a single, fully upgradeable instrument that can meet the differing demands of users, according to the company. Script to download file from labtech cloud server help labtech 11 been having issues with one of my old scripts to download a file from my labtech server which is being hosted in the cloud from labtech. Cellbased assays to determine the inhibition ic 50 of nonmet kinases by ly2801653 cellbased assays antiproliferative or inhibition of phosphorylation of target kinase were performed to evaluate the. We have access to and expertise using ge incell analyzer 2200, ge incellanalyzer 6000, ttp labtech acumen explorer, bd pathway. Discovery of a novel 2,3,11,11atetrahydro1hpyrazino1,2.
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